Asst. Prof. Dr. Şerife AYAZ GÜNER
I received my B.S. and MSc from Middle East Technical University, Turkey. I moved to US for Ph.D. training at the University of Wisconsin – Madison, where I obtained a Ph.D. in Cellular and Molecular Biology Program under the mentorship of Dr. Wei Xu. During my graduate work, I focused on estrogen receptor palmitoylation and estrogen dependent rapid signaling pathways. Because of my long term interest in PTMs and cell signaling, I joined the Ying Ge’s group as a post-doctoral researcher in January 2014. I applied a variety of different separation and mass spectrometry techniques to investigate heart disease-related changes in protein expression and post-translational modifications. Ultimately, I aim to combine my knowledge and skills in cell biology with proteomics techniques to contribute to the understanding of disease progression and development of new therapeutic targets.
Contact Information:
E-mail: serife.ayaz@agu.edu.tr
Phone: +90 352 224 88 00 (Ext: 7358)
Projects
Identification of Trueperella pyogenes Pyolosin-specific Nanobodies Using Proteogenomic Approaches, TÜBİTAK 1001 (121O664), Coordinator: Şerife AYAZ GÜNER.
Determination of New Chimeric Antigen Receptor (CAR) Targets by Investigating B Cell Acute Lymphoblastic Leukemia (B-ALL) Surface Proteome, TÜBİTAK 1001 (119S457), Coordinator: Servet Özcan, Researcher: Şerife AYAZ GÜNER.
Publications
Hitit M, Özbek M, Ayaz-Guner S, Guner H, Oztug M, Bodu M, Kirbas M, Bulbul B, Bucak MN, Ataman MB, Memili E, Kaya A. Proteomic fertility markers in ram sperm. Anim Reprod Sci. 2021;235:106882.
Acar MB, Ayaz-Guner S, Gunaydin Z, Karakukcu M, Peluso G, Di Bernardo G, Ozcan S, Galderisi U. Proteomic and Biological Analysis of the Effects of Metformin Senomorphics on the Mesenchymal Stromal Cells. Frontiers in Bioengineering and Biotechnology. 2021;9;10.3389/fbioe.2021.730813.
Acar MB, Aprile D, Ayaz-Guner S, Guner H, Tez C, Di Bernardo G, Peluso G, Ozcan S, Galderisi U. Why Do Muse Stem Cells Present an Enduring Stress Capacity? Hints from a Comparative Proteome Analysis. International Journal of Molecular Sciences. 2021; 22(4):2064.
Acar MB, Ayaz-Güner S, Di Bernardo G, Güner H, Murat A, Peluso G, Özcan S, Galderisi U. Obesity induced by high-fat diet is associated with critical changes in biological and molecular functions of mesenchymal stromal cells present in visceral adipose tissue. Aging (Albany NY). 2020;12:10.18632/aging.202423.
Ayaz-Guner, S.; Alessio, N.; Acar, M.B.; Aprile, A.; Ozcan, S.; Di Bernardo, G.; Peluso, G.; Galderisi, U. A comparative study on normal and obese mice indicates that the secretome of mesenchymal stromal cells is influenced by tissue environment and physiopathological conditions,Cell Commun Signal 18, 118 (2020).
Brown, K. A.; Chen, B.; Guardado-Alvarez, T. M.; Lin, Z.; Hwang, L.; Ayaz-Guner, S.; Jin, S.; Ge, Y. A Photocleavable Surfactant for Top-Down Proteomics, Nature Methods 16, 417-420 (2019)
Martin-Garrido A, Biesiadecki BJ., Salhi HE., Shaifta Y., Dos Remedios CG., Ayaz-Guner S., Cai W., Ge Y., Avkiran M., Kentish JC. Monophosphorylation of cardiac troponin-I at Ser-23/24 is sufficient to regulate cardiac myofibrillar Ca2+ sensitivity and calpain-induced proteolysis. J Biol Chem. 293(22):8588-8599 (2018)
Hwang L.; Guardado-Alvarez T.; Ayaz-Guner S.; Ge Y.; Song J.; A Family of Photolabile Nitroveratryl-Based Surfactants That Self-Assemble into Photodegradable Supramolecular Structures, Langmuir, 2016, 32 (16), 3963–3969
Cai W.; Guner H.; Gregorich Z.R.; Chen A.J.; Ayaz-Guner S.; Peng Y.; Valeja S.G.; Liu X.; Ge Y.; MASH Suite Pro: A Comprehensive Software Tool for Top-Down Proteomics, Molecular & Cellular Proteomics, 2016, 15 (2), 703-714.
Yu D; Peng Y.; Ayaz-Guner S.; Gregorich Z.R.; Ge Y.; Comprehensive Characterization of AMP-Activated Protein Kinase Catalytic Domain by Top-Down Mass Spectrometry, J. Am. Soc. Mass Spectrom., 2016, 27 (2), 220-232.
Ayaz-Guner, S*.; Chen, Y*.; Peng, Y.; Lane N. M.; Locher M. R.; Kohmoto T.; Larsson L.; Moss R. L.; Ge, Y.; Effective top-down LC/MS+ method for assessing actin isoforms as a potential cardiac disease marker, Analytical Chemistry, 2015 87 (16), 8399-8406 .(* Equal contribution)
Hwang L.; Ayaz-Guner S.; Gregorich Z.R.; Valeja S.; Chang Y.; Cai W.; Jin S.; Ge Y.Highly Specific Enrichment of Phosphoproteins Using Functionalized Multivalent Nanoparticles, J. Am. Chem. Soc., 2015, 87, 5363-5371.
Ayaz-Guner, S.*; Peng, Y.*; Yu, D.; Ge, Y. Top-down mass spectrometry of cardiac myofilament proteins in health and disease, Proteomics Clin. Appl., 2014, 8(7-8), 554-568. .(* Equal contribution)
Zhao, Z., Wang, L., Wen, Z., Ayaz-Guner, S., Wang, Y., Ahlquist, P., and Xu, W. Systematic analyses of the cytotoxic effects of compound 11a, a putative synthetic agonist of photoreceptor-specific nuclear receptor (PNR), in cancer cell lines, Plos One, 2013, 8(9):e75198.
Guy, M. J.; Chen, Y.; Clinton, L.; Zhang, H.; Zhang, J.; Dong, X.; Xu, Q.; Ayaz-Guner, S.; Ge, Y. The impact of antibody selection on the detection of cardiac troponin I, Clin. Chim. Acta., 2013, 420, 82-88.
Zhang, J.; Ayaz-Guner, S.; Dong, X.; Xu, Q.; Guner, H.; Ge, Y. Phosphorylation, but not alternative splicing or proteolytic degradation, is conserved in human and mouse cardiac troponin T, Biochemistry, 2011, 50, 6081-6092.
Ayaz-Guner, S.; Zhang, J.; Li, L.; Walker, J. W.; Ge, Y. In vivo phosphorylation site mapping in mouse cardiac troponin I by high resolution top-down electron capture dissociation mass spectrometry: Ser22/23 are the only sites basally phosphorylated, Biochemistry, 2009, 48, 8161-8170.